ASSAY DEVELOPMENT

Arcxis can offer Tentacle Probe(tm) technology for assay development, under a contract basis. We have successfully completed assay development for other collaborators see:

Tentacle Probe Offers Assurance for Anthrax Detection

If you are experiencing issues with your current technology, encountering false positives in the detection of difficult insertion and/or deletion sequences, we would be interested in working with you to help you develop a successful assay that is based on Tentacle Probe Technology. Contact us at: information@arcxis.com

No False Positives: The overall footprint of the Tentacle Probe™ includes a capture sequence and the interrogation sequence that spans a relatively large part of the target genome (35 – 45 bp) compared to other dual labeled probes. This larger sequence is particularly useful when designing probes that are able to distinguish between close families of homologous genes or pseudo genes, thereby eliminating the chances of false positives.

Difficult sequence insertions & deletions: Additionally, the self-complimentary part of the probe can be designed to be longer, conferring a tighter binding than is normally encountered with conventional dual labeled probes and therefore allowing for higher DNA melting temperatures. The ability to perform the PCR at higher temperatures during the detection stage can have the effect of opening out any loop structures or similar tertiary structures on the genomic DNA. This makes the probes particularly suitable for the detection of difficult insertions and deletions (IN/DELS) where the use of conventional probes would fail, or lead to inconclusive results.